Correlation and clinical significance of methyltransferases SETDB1 and SPG20 methylation in lung adenocarcinoma

Objective: The incidence of primary lung adenocarcinoma is increasing year by in worldwide, and it has surpassed squamous cell carcinoma to become the most common pathological type cancer. Advances medical technology have made overall survival rate cancer patients improve, but still very low. In study, we investigated methyltransferase SETDB1 expression methylation level SPG20, analyzed correlation its clinical significance pathogenesis adenocarcinoma. Methods: 60 normal tissues were selected.The SPG20 mRNA was examined RT-qPCR; Protein determined immunohistochemistry(SP) immune protein imprinting(Western blot);Methylation gene pyrosequencing. Correlation between levels both case characteristics analysed statistics. Western blot detection cells bronchial mucosal epithelial cells.The small interfering RNA transfected into A549 liposome-mediated method, detected RT-qPCR Wesrern blot, Results:Relative significantly lower tissue than tissue, while relative higher tissue. showed cancerous difference statistically significant. a with positive correlation; are closely related TNM stage, differentiation, lymph node metastasis vitro experiments that highly expressed, lowIn cells, low high cells. interference could reduce decreased, increased. Conclusion:There significant methyltransferases adenocarcinoma, may be upstream molecule methylation, working together promoting occurrence development